Molecular Analyses of Citrus tristeza virus Subisolates Separated by Aphid Transmission

Inefficient Transmission of Citrus Tristeza Virus from Grapefruit by Single Brown Citrus Aphids

Six severe and six mild Florida isolates of citrus tristeza virus (CTV) were used to evaluate the transmission efficiency of the virus from grapefruit seedlings by single brown citrus aphids (Toxoptera citricida Kirkaldy) (BrCA) from colonies initiated by aphids obtained from citrus groves in Fort Pierce, Fla. The transmission rate to 2120 receptor plants [‘Mexican’ lime (Citrus aurantifolia)] ...

Citrus tristeza virus-host interactions

Citrus tristeza virus (CTV) is a phloem-limited virus whose natural host range is restricted to citrus and related species. Although the virus has killed millions of trees, almost destroying whole industries, and continually limits production in many citrus growing areas, most isolates are mild or symptomless in most of their host range. There is little understanding of how the virus causes sev...

Molecular Virology and Pathogenicity of Citrus tristeza virus

Sequences of Citrus tristeza virus separated in time and space are essentially identical.

The first Citrus tristeza virus (CTV) genomes completely sequenced (19.3-kb positive-sense RNA), from four biologically distinct isolates, are unexpectedly divergent in nucleotide sequence (up to 60% divergence). Understanding of whether these large sequence differences resulted from recent evolution is important for the design of disease management strategies, particularly the use of genetical...

The Complex Genetics of Citrus tristeza virus

The 2000 x 11 nm long bipolar flexuous filamentous particles of Citrus tristeza virus (CTV) (genus Closterovirus, family Closteroviridae) (Figure 1) contain a single-stranded positive-sense RNA genome of 19.3 kb, which is encapsidated in two different capsid proteins that coat the opposite ends of the virions [1, 2]. CTV is the largest identified RNA virus infecting plants and the second larges...